1/3/2023 0 Comments Gms stain![]() Silver nitrate forms insoluble silver phosphate with phosphate ions this method is known as the Von Kossa Stain. These reduce silver solution to metallic silver after being exposed to the stain that contains a reductant, for example hydroquinone or formalin. These reduce silver solution to metallic silver after formalin fixation. Many variables can influence the colour intensity and every protein has its own staining characteristics clean glassware, pure reagents, and water of highest purity are the key points to successful staining. Ĭlassical Coomassie brilliant blue staining can usually detect a 50 ng protein band silver staining increases the sensitivity typically 50 times. The technique has been extended to the study of other biological macromolecules that have been separated in a variety of supports. ![]() Silver staining was introduced by Kerenyi and Gallyas as a sensitive procedure to detect trace amounts of proteins in gels. ![]() Although the exact chemical mechanism by which this occurs is unknown, Golgi's method stains a limited number of cells at random in their entirety. Camillo Golgi perfected silver staining for the study of the nervous system. ![]()
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